I'm in Warhammer, I just want to grow mushrooms

Chapter 24: Purification of Stupidity

Nine o'clock in the evening, in a simple and cramped residence.

The house is filled with a large amount of materials related to cultivating fungi, and it is more crowded than before.

Li Nuo sat in the chair, saying nothing, immersed in the work of simulating the domestication of stupid mushrooms.

[On the eighth day, all samples were contaminated by bacteria. This simulation failed.]

Failed again.

Very meow.

Are the vitality of the fungi in the Warhammer world so strong?

It's outrageous that even alcohol and sunlight can't kill the fungi on the stupid mushroom.

Even more outrageous is the stupid mushroom itself.

This thing looks similar to ordinary poisonous mushrooms, but it is surprisingly durable and can even grow in alcohol.

It’s so outrageous that his mother opened the door for outrageous things. It’s so outrageous.

Li Nuo took out a milk mushroom and started to eat it with a smacking sound to relieve the depression in his heart.

It seems that we need to change the way of domestication.

Now that I have a lot of money, I might as well spend it and buy the sterile instruments needed for the dilution spread plate method.

Li Nuo crawled under the bed, took out a large stack of ration cards, hid them under his clothes and went out.

It was 11 o'clock in the evening when I got home again.

In addition to the fatigue from a busy day, Li Nuo's face also showed a slight pain in his flesh.

He had a black leather suitcase with a mechanical combination lock in his hand.

In order to buy the things in the suitcase, he used up nearly three hundred ration cards, which could be described as a huge loss.

Hopefully this simulation will be successful.

Put down the suitcase, unlock the combination lock, and a glass container appears in front of you.

The container contains sterile test tubes, sterile droppers, sterile Erlenmeyer flasks, sterile applicators, sterile culture medium plates, and a pair of gloves for aseptic operations in the container.

This equipment is called a portable sterile chamber. It features simplicity and is not equipped with ultraviolet rays or fans.

It was a second-hand item that Li Nuo bought from the market. The original owner was an unlicensed wild pharmacist.

This person recently invented a special medicine for treating trauma and made a lot of money. He bought a lot of new equipment and sold his used things at a discount. This way, Li Nuo got a good deal.

Portable sterile chambers are not ideal experimental equipment.

What Li Nuo really wanted to buy was a clean bench.

It can create a sterile environment by forming a positive pressure inside and sterilizing with ultraviolet light.

However, the clean bench is too expensive, so Li Nuo had to settle for the next best thing and use a portable sterile chamber as a substitute.

Normally, before using a sterile chamber, you must first ensure that there are no bacteria in the surrounding environment.

Otherwise, when the sample is placed in, the sterile chamber will be contaminated by bacteria in the environment.

The current accommodation obviously does not meet the conditions for use in a sterile warehouse.

But it's okay.

The system helps a lot in this regard.

Li Nuo reached out and pressed on the sterile chamber, and the system interface popped up.

【Discover the portable sterile chamber.】

【Absorbing it can permanently upgrade the fungus simulation function. 】

【May I ask if you have absorbed it? 】

Li Nuo silently said "absorb".

"call out----"

After the vague prompt sound, the sterile chamber disappeared out of thin air and the suitcase was empty.

【The fungus simulation function has been successfully upgraded. 】

【New simulation container: portable sterile warehouse. 】

[From now on, you can select this container for simulation without preparing additional containers. ]

Li Nuo sat up straight:

“Begin simulating the removal of bacterial contamination.”

【The simulation is about to begin. 】

【Please select the experimental subject. 】

"Spores of the stupid mushroom."

【Experimental subjects have been set.】

【Please select a container. 】

“Use a sterile warehouse and the sterile props that come with it.”

【Container setting completed. 】

【Please select the culture medium and nutrient solution. 】

Li Nuo gestured on the table where the raw materials were placed:
"Use the formula I used in my previous simulation to make the culture medium and nutrient solution for this simulation."

【Please set the simulation parameters. 】

"Use the dilution spread plate method to separate and purify spores contaminated by other bacteria. For parameters such as temperature, humidity, and light, refer to the previous simulation records."

【Setting completed.】

【Start simulation.】

[On the first day, you used the spores of the stupid mushroom and a triangular flask filled with sterile water to make a 10% bacterial suspension.] [You used a 10-fold serial dilution method to obtain a 10% bacterial suspension.]

【You use a sterile spreader to spread the diluted bacterial suspension onto a sterile solid culture medium plate. 】

【You have obtained 10 experimental samples. 】

【You observe the sample at rest. 】

【From the second to the fifth day, the microorganisms on the culture medium plate grow and multiply to form colonies. 】

[You use the fungus analysis function to determine the types of microorganisms in the colony. ]

[You found that all 10 experimental samples were contaminated with bacteria. ]

[The least contaminated sample had a colony composed of 33% miscellaneous fungi and 67% of the mycelium of the stupid mushroom.]

【At this time, you decide? 】

The principle of the dilution spread plating method is well understood.

Suppose that among 100 microorganisms, there are 99 miscellaneous bacteria and 1 fungus that you want.

We used the dilution method to mix these 100 microorganisms with a large amount of sterile water to obtain a bacterial suspension.

Divide the bacterial suspension into 100 equal portions of bacterial suspensions with lower microbial concentrations.

If you are lucky, one of them will be free of bacteria.

By taking this part out separately and cultivating it intensively, you can obtain fungi that are not contaminated by other bacteria.

This is the general process of the dilution plate method.

What if every bacterial suspension contains foreign bacteria?
Don't panic.

Continue to dilute, divide evenly, and perform the dilution and plating method until a bacterial suspension free of contamination by other bacteria appears.

This method of removing bacteria is very cumbersome and often takes experimenters several months.

But it is undeniable that as long as you persist, you will definitely be able to obtain uncontaminated bacterial suspension.

Li Nuo instructed the system without much hesitation:
"I selected the least contaminated sample as the new experimental subject and used the dilution plate method again for separation and purification."

[On the fifth day, you use the dilution plate method on a new experimental subject and obtain 10 experimental samples.]

[On the tenth day, you used the fungus analysis on the samples and found that the least contaminated sample contained about 30% of miscellaneous fungi, and the rest was the mycelium of the stupid mushroom.]

【At this time, you decide? 】

Well, that's good. The pollution level has dropped from 33% to 30%. That's good news.

"I decided to repeat my previous actions and continue to isolate and purify the least contaminated samples."

[On the tenth day, you obtain 10 new experimental samples.]

[On the fifteenth day, the least contaminated sample had about 28% of foreign bacteria. What do you decide at this time?]

"continue."

On the 26th day, the least contaminated sample had % of foreign bacteria.

"continue."

【Day 25…】

【Day 30…】

【Day 35…】

The number of simulated days rose rapidly.

Li Nuo sat on the chair and repeated his instructions over and over again.

Soon, it was midnight, but there were still 2% of the bacteria that had not been separated.

Li Nuo kept his composure and didn't give up.

【Day 160.】

【Day 380.】

【Day 620.】

The remaining bacteria were so stubborn that the system performed no less than a hundred separation and purification operations, but there were still less than 0.02% of the bacteria in the sample.

The bacteria in the Warhammer world are too stubborn!

Finally, when the simulation reached the terrifying 1,200th day, Li Nuo finally got the result he wanted.

[On the 1,200th day, you obtained a sample that was not contaminated by bacteria.]

[You discovered the mycelium of the stupid mushroom from the sample and cultivated it.]

[On the 15th day, you harvested the samples and obtained domesticated stupid mushrooms.]

【Congratulations! 】

【This simulation was successful! 】

[The simulation plan has been recorded. ]

[Can I consume simulation materials to obtain 15 domesticated stupid mushrooms? ]

"Finally succeeded! If this had happened before I traveled through time, my hair would have turned white!"

Li Nuo was so excited that he said "confirm" silently.

There were 15 more stupid mushrooms in the fungus warehouse, all snow-white without a trace of other colors.

Li Nuo glanced at the clock on the wall.

Two o'clock midnight.

Try the effects of brain freeze now and then go to sleep.

Otherwise, I might fall asleep at the excavation site during the day.

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(End of this chapter)