I'm in Warhammer, I just want to grow mushrooms

Chapter 186: Not Selling the Imperial Ditch, But Enjoying the Imperial Blessings

【Day 1】

[You used Chaos Contract Yeast and Archaic Crystal Fungus as experimental subjects.]

You prepared 100 copies of bacterial suspensions containing two bacterial colonies and used the dilution spread plate method to prepare culture plates.

【You have obtained 100 experimental samples. 】

【Day 2 to Day 15. 】

[95 samples showed serious mutations, which were manifested as bacteria contamination and deep signs of chaos and corruption. Please see the observation image of the holographic microscope. At this time, what do you decide? ]

A holographic image appeared before my eyes, and the content could only be described as unbearable to the eyes.

The normal bacterial colony turned into a hideous appearance, either growing flesh and blood limbs or long beards covered with black hair.

These experimental samples are definitely not usable.

Li Nuo silently thought in his heart:
"I decided to discard and render harmless the mutant samples."

[95 mutant samples have been rendered harmless. You continue to cultivate the remaining 5 samples.]

[Day 16 to Day 30]

[The remaining 5 samples mutated. What do you decide at this time? ]

"I've decided to call it quits and abandon this simulation."

Li Nuo was not surprised by the result. This was just the first attempt and failure was normal.

He paced in his private laboratory, recalling in depth the knowledge he had learned about microorganisms.

Gradually, more knowledge points about "antagonism" emerged in my mind.

Li Nuo nodded slowly, realizing where his problem lay.

In the past six months, he had spent most of his time conducting genetic simulations, and the experimental procedures in the field of microorganisms had become so unfamiliar that he had overlooked several key points.

After clarifying his experimental ideas, Li Nuo sat down at the table and started a new simulation.

This time, he chose manual rather than automatic operation and personally controlled every step of the experiment, just like when he first traveled through time.

"System, start the antagonism experiment."

【Please set the experimental subjects. 】

"Target strain: Chaos Contract Yeast. Antagonistic strain: Archaic Crystalline Fungus."

【Please set the culture medium. 】

Li Nuo narrowed his eyes and thought for a moment:
"Use PDA medium to cultivate Archaic Crystal Fungi, and use LB medium to cultivate Chaos Contract Yeast. The raw material of LB medium is the blue-purple ghost mushroom."

The main components of PDA medium are potatoes, glucose and agar.

When he first came here, Li Nuo used synthetic starch and orange sugar instead of potatoes and glucose.

Now that he was rich, he bought authentic potatoes and glucose from the agricultural world, so the agar medium he used at this time was the real PDA medium.

LB medium is also known as lysing broth. Its main ingredients are peptone, yeast extract and sodium chloride. It can promote the growth of miscellaneous bacteria and further expand the contamination of miscellaneous bacteria.

Chaos corruption is not bacterial contamination.

When expanding Chaos Corruption, materials related to negative emotions should be used instead of traditional LB culture medium raw materials.

Therefore, Li Nuo specifically asked the system to use blue-purple ghost mushrooms to make LB culture medium.

This ghost mushroom carries very strong negative emotions, and emotions are Chaos' favorite "dessert", which should be able to promote the further expansion of Chaos Corruption.

【The culture medium is set. 】

【Please set the instrument and environmental parameters. 】

Li Nuo took a deep breath and said what he had prepared in advance:

“Use sterile operating table, constant temperature incubator, centrifuge, chromatograph, holographic microscope, and single molecule detector as experimental instruments. The environmental parameters refer to the conventional experimental plan.”

【The instrument and environmental parameters have been set. 】

【Please set up the reagents. 】

Reagents are used to test whether Antagonism has an effect on Chaos Corruption.

A material that is extremely sensitive to Chaos Corruption must be used as a reagent.

So what meets the requirements?

The first thing Li Nuo thought of was the Mark of Purity.

This item is a must-have prop for every Space Marine in the 40k era. It is made of milky white parchment and red paint print.

When encountering Chaos Corruption, the parchment will burn, alerting the Space Marines with flames and smoke.

But it’s the 30k era now, and the Emperor hasn’t even sold hooks on the golden toilet yet, so where can I find the Mark of Purity?

Li Nuo folded his arms, frowned, and began to search in the fungus warehouse.

After looking around, Li Nuo's eyes lit up slightly.

He realized he had reached a dead end.

In fact, as long as the life structure of a certain fungus is fragile enough, it can be used as a reagent.

Being fragile enough means being easy to change.

If something is easily changed, it is easily corrupted.

Assuming that the Chaos Pollution required to corrupt other substances is 1, then the amount of Chaos Pollution required to corrupt this fungus may be only 0.01.

If you understand this, you will have a lot of fungi to choose from.

"The system uses spores of Saccharomyces cerevisiae and sterile water to make the reagents."

The cell wall of blastospores is very thin and the protective structure is very simple, which is easily affected by the external environment and chaos.

【Reagent setting completed.】

【Start simulation.】

[On the first day, you inoculate the Archaic Crystal Fungus in the center of the PDA plate, with a cake diameter of 1 mm. You inoculate Chaos Contract Yeast around the Archaic Crystal Fungus 5 cm away from the Archaic Crystal Fungus to form an inhibition zone.]

【Room temperature 28 degrees Celsius, relative humidity 80%, no light, sterile environment. 】

[On the 5th day, you measured the radius of the inhibition zone and calculated the inhibition rate. The result was 13%, indicating that the Archaic Crystal Fungus has an antibacterial effect on Chaos Contract Yeast.]

[On the 10th day, you calculated the inhibition rate again and the result was 26%, indicating that the inhibition effect increased over time. ]

[On the 15th day, the fungus cake came into contact with the antibacterial circle, and the ancient crystalline fungus mutated. At this time, what do you decide? ]

Mm-hmm, very good.

Li Nuo crossed his arms, nodded with satisfaction, and thought to himself that it was just as he expected.

The Archaic Crystalline Fungus cannot directly fight against the Chaos Contract Yeast. After the two come into contact, the Archaic Crystalline Fungus has a 100% chance of being corrupted.

This is sufficiently proven by two experiments.

Then why can the ancient crystalline fungi have an antibacterial effect on chaos contract yeast?
According to Li Nuo's experience, this is due to metabolites.

The next step is to analyze the antagonistic activity of the metabolites.

"System, start a new simulation experiment. All experimental parameters remain unchanged, but this time, obtain the extract of the ancient crystalline fungus metabolites first."

[Day 1 to Day 7]

[You ferment the ancient crystal fungus at room temperature of 28 degrees Celsius, filter the fermentation liquid using a 0.22μm thick filter membrane, and obtain a sterile fermentation product. ]

[You extract the fermented product with ethyl acetate and concentrate it by rotary evaporation to obtain a fermented product extract. ]

【At this time, you decide? 】

"The antibacterial rate of the extract was determined using the filter paper diffusion method."

[On the 7th day, you use Chaos Contract Yeast and sterile water to make a bacterial suspension and spread it evenly on the LB plate. ]

You smear the extract on a filter paper, stick it on an LB plate, and incubate it at room temperature at 37 degrees Celsius for 24 hours.

[On the 8th day, you measured the inhibition zone and found that the inhibition rate was 55%, indicating a significant antibacterial effect.]

Very good, very good, the metabolites of the ancient crystalline fungi do have antibacterial effects.

This is easy to handle.

“Isolate the different components in the metabolites and test their antibacterial effects separately. Select the metabolite with the most significant antibacterial effect and use a mass spectrometer to obtain its molecular formula.”

[Day 9 to Day 15]

[You have determined through a large number of experiments that the antibacterial effect comes from an unknown metabolic component. You use a mass spectrometer to obtain the molecular formula of this substance, and the results are as follows...]

A string of molecular formulas appeared in the field of vision, so complex that it was dazzling.

Li Nuo aimed at it and threw out the analytical fungus to obtain relevant information.

【Name: A metabolite that can inhibit chaos corruption.】

【Analysis results:】

[The antibacterial rate of this metabolite on Chaos Contract Yeast reaches 99%. ]

[The metabolic mechanism for the production of this metabolite is determined by the gene fragment of the Archaean crystalline fungus: Frag015754. ]

With the number of the gene fragment, gene editing can be performed.

Li Nuo starts fungal gene simulation.

"System, use the Archaic Crystal Fungus as the experimental subject, scan its genetic samples, and find the genetic fragment numbered Frag015754."

[Scan completed. 】

【Gene fragment: Frag015754. 】

【Function: This fragment is related to some of the metabolism of Archaean crystalline fungi. 】

[When the ancient crystalline fungi absorb the subspace energy with emotions, they will secrete a specific metabolite. ]

【Affected by this fragment, the secretion efficiency of metabolites is 1%. The secretion efficiency can be improved through gene editing. 】

Li Nuo took a deep breath and smiled:
"Pause the current fungal gene simulation, start automated fungal gene simulation, and look for genetic coding that improves secretion efficiency." [You paused the "Bone Drinker RNA Vaccine R&D Project."]

【Project progress has been saved. 】

【Start a new automation simulation. 】

【Day 1】

【...】

When it comes to automatic simulation, time flies by like running water.

In a blink of an eye, 26 days and nights were spent in busy work.

That evening, when Li Nuo was preparing to enjoy dinner, the automatic simulation produced results.

【Day 62407713】

【You make your 1622600538th attempt.】

You use the CRISPR-Cas9 design tool available at the Imperial Medical Department.

[You extracted a 91-nucleotide DNA sequence from the Archaean crystalline fungus, whose 52' end complemented the experimental object, and the 21' end bound to the Cas9 protein to form a new gene fragment.]

[You knock out the gene fragment corresponding to the experimental subject and replace it with a new gene fragment.]

[Fungus simulation experiments have confirmed that the efficiency of the Archaic Crystal Fungus in secreting specific metabolites has been greatly increased to 100%, and the secretion of other metabolites has been stopped. ]

“Hiss—it increased to 100% all at once?! Is this fungus still the ancient crystal fungus?”

Li Nuo walked into the private laboratory, sat down at the table, stared at the colony on the holographic microscope image, and started analyzing the fungi.

The analysis results almost made him jump to the ceiling!

[Name: Archaic Crystalline Fungi (70% Reversion)]

[Type: Ancient Saint Creation]

【Analysis results:】

[The degree of atavism increased by 10%, and the output of subspace energy single crystals per Terra hour increased from 1 kg to 5 kg. 】

[In addition, this fungus can be used to remove Chaos Corruption. But please note that the process of removing Chaos Corruption can only be performed in the system's fungus simulation function and cannot be performed in the real universe. ]

Oh my God!
This is so unexpected!

It’s a double happiness!

"Emperor, I wrongly blamed you. If you hadn't sold out to the Chaos Demon, I wouldn't have obtained the Chaos Contract Yeast, nor would I have thought of purifying it, let alone promoting the further reversion of the Archaic Crystalline Fungus through gene editing.

"I'm sorry, Emperor. Your ditch is a good thing. You need to sell more. The more the better. This way I can take back more good things belonging to the Ancient Saints from Chaos. Wakaka~"

Li Nuo was so excited that his body was shaking, and he immediately started the experiment of removing the chaos corruption.

After 10 minutes, the system text is refreshed.

[You have removed the Chaos Corruption of the Chaos Contract Yeast and completed the purification work.]

[Chaos Contract Yeast becomes Sealing Yeast, which can seal the subspace entity in the real universe through psychic fermentation.]

[This yeast has a complex life structure, but has outstanding gene expression capabilities and can be used as an expression vector for gene editing. ]

[Affected by gene purity, the expression effects of psychic fermentation and gene editing are unstable, which can be improved by improving gene purity. 】

[The current gene purity is unknown and will be unlocked after obtaining the gene template. ]

[When the genetic purity reaches 90%, the fungus is domesticated. ]

It’s OK, everyone. Chaos Contract Yeast has transformed into Sealed Yeast, and the new gene expression vector has been completed.

Improving genetic purity and the like can be put off until later, there is no rush.

"I remember there was a kind of Chaos-corrupted fungus in the Fungal Warehouse..."

Li Nuo rummaged through the fungal warehouse and clicked on the panel of psychic mold.

Its panel description is as follows:

【Name: Unknown pathogenic bacteria】

【Analysis results:】

[This psychic mold was transformed by Nurgle's hand. Its toxicity is only effective on a very small number of plants and is harmless to humans. ]

[The biggest feature of this object is the "subspace superposition state". It can be observed by the physical universe, but cannot be captured by the physical universe.]

[When you try to inoculate and cultivate it, it will immediately return to the subspace, preventing you from conducting in-depth research on it. ]

This psychic mold comes from diseased whiteback tobacco leaves.

Its bacterial ability is very strong, which once made Li Nuo very excited.

[Bacteria ability: Subspace walking]

【Ability description:】

[You fade out of the physical universe and enter the surface of the subspace. In the physical universe, you are invisible and can pass through walls. You have no physical collision volume, can ignore obstacles, and are immune to any damage from the physical universe. However, you will emit spatial signals from the subspace, and then be discovered by people and objects with psychic recognition abilities. ]

[Unlock requirement: 100 skill point. ]

[After successful taming, the unlocking requirement is reduced to 1 skill point. ]

[The taming process will not alert Nurgle. 】

[Please do not forcefully unlock and use this fungus's ability before taming is complete! Otherwise, terrible consequences will occur! ]

Li Nuo had no way to tame psychic mold before, but now it’s different.

Ancient crystalline fungi, start!
10 minute later.

[You have successfully removed the chaos corruption from the psychic mold and completed the taming work. ]

[After taming, the psychic mold becomes ghost mold. 】

[The panel of this mold is as follows:]

【Name: Ghost mold】

【Analysis results:】

[An ancient mold handed down from the dark technological age. 】

[Its mycophenolate moxibustion can be used to prepare an "invisibility potion" that grants the drinker temporary "subspace walking" abilities.]

[Bacteria ability: Subspace walking. 】

[Unlock requirement: 1 skill point. ]

Without saying a word, Li Nuo unlocked the fungus ability that he had been coveting for a long time, quickly cultivated 100 tons, and then activated the subspace walking.

The private laboratory faded from his sight and became a grayish-white picture. The buzzing sound of the experimental instruments disappeared, and the surroundings became exceptionally quiet.

Li Nuo tried to stretch out his right hand towards the wall, and watched with wide eyes as most of his arm passed through the wall, but he did not feel any discomfort at all.

"Awesome! Now I can walk through walls!"

Look at the three Black Knights in the corner.

A hazy white light appeared on their body surface.

As he got closer, Li Nuo felt a distinct sense of frustration, as if the white light was pushing him away.

This is the psychic shield that comes with the Warp single crystal. It only works on Warp creatures and Warp energy, giving the Black Knight unparalleled resistance to Warp space.

Warp walking is achieved through the warp and is also within the range of the shield.

Li Nuo stretched out his arm to the Black Knight and touched the white light. He found that this thing looked like a layer of light curtain, but it had the touch of steel and was truly indestructible.

Li Nuo nodded slightly, extremely satisfied with the single crystal's subspace resistance.

He ended his walk in the warp and turned his head to look at the psychic reading table on the wall.

A sharp curve bulged upward, and the reading level at the highest point reached an astonishing gamma level!

It turns out that warp walking is a psychic power that only gamma-level psychics can master. It's so terrifying!
The walls of this laboratory were installed with anti-psychic lead plates to prevent psychic fluctuations on both sides from spreading to the other side. There was no need to worry about the little psychic experiment just now being detected by people and instruments outside.

Li Nuo calmed his mind, suppressed his excitement, and operated on the system interface.

Fungal gene simulation and fungal cultivation simulation can be carried out simultaneously.

Cultivation simulations are used to produce single crystals and replenish stocks of commonly used fungi.

As for genetic simulation, this is what Li Nuo thought.

First develop an RNA vaccine for Bone Drinkers, then use sealed yeast as an expression vector to produce Bone Drinker spores with a genetic purity of more than 90%, unlocking more bacterial capabilities.

The RNA vaccine is a mixture of part of the RNA of the fungal phage virus, the fermentation product of reverse parasitic yeast, and the self-developed nucleotide repair fluid.

It has a 100% kill rate, is far safer than previous generation vaccines, and only requires one vaccination to provide lifelong immunity to the Bone Drinker parasite.

The self-developed nucleotide repair fluid is low-cost, with each bottle costing less than 1 Throne Coin, but the repair effect on the gene chain is limited to mutations caused by fungal infection.

After the RNA vaccine and bone drinker fungi are unlocked, genetic simulation will be used to induce the ancient crystalline fungi to further revert to their ancestral form.

The two fungal simulations are progressing hand in hand, and a bright future is just around the corner.

After the meal, Li Nuo sent away Tido who came to eat for free, and then started his daily work as the Lord of the Green-skinned Palu.

The archived briefing reminded him that it was time to log into Blood Crow Boy's account and deal with the 13 Dark Angels Inner Circle veterans.

[Archive 8: Blood Crow Boss - Kron.]

Archived briefings:

[After 26 days of subspace voyage, the frigate is about to reach its destination. ]

[The anesthetics you delivered to the veterans in the inner ring have tempered their will and drug resistance.]

[Now they have almost adapted to the presence of anesthetic ingredients and are largely immune to such ingredients. ]

[Go online before the Inner Circle veterans go berserk! Otherwise, the Blood Crow boys will die! ]

(End of this chapter)