As a mage, I just want to pursue the truth

Chapter 76 Dawn
"In agriculture, a paper is just a summary of your work. The essence of evaluating the quality of your work is the positive impact your research results have on agriculture. Publishing a paper is just a byproduct of that essence."

"I have no way to convince you to believe my method now. The cabbage matures in about 40 days. If you take good care of the crops, the final results will prove that I am right."

Dong Wenxu didn’t know where Jiang Yan’s confidence came from. He just hoped that the work during this period was not in vain.

However, Dong Wenxu was able to experience joy in the planting process. He sometimes thought that the Chinese people's farming genes were too strong.

Farming is an instinct deeply rooted in the genes of the Chinese people. After obtaining the standard magic brain, Jiang Yan continued to devote himself to agricultural research.

Jiang Yan can sense life, so Simmons also has this ability. The characteristics given by the standard magic brain are the same.

Simmons joined Pfizer's special drug research and development team, and American hopes he can work magic.

The researchers identified twelve proteins as potential targets.

They can be roughly divided into two categories: the first is structural proteins, including spike proteins, envelope small membrane proteins, membrane proteins, nucleocapsid proteins and another category of proteins called non-structural proteins.

The non-structural proteins mainly include protease (M pro), papain, nsp13: helicase, nsp12: RNA-dependent RNA polymerase, nsp14: N-terminal exoribonuclease and C-terminal guanine-N7 methyltransferase, nsp15: uridylate-specific endoribonuclease, nsp16: 2'-O-methyltransferase and nsp10.

The virus is responsible for the replication and packaging of the viral core material into the capsid protein coat.

Blocking any of these proteins results in a halt in the virus's ability to replicate or to package its RNA into a protein coat.

Among a total of 12 proteins identified as targets for controlling CoV; 3CLpro was identified as one of the most important targets due to its critical role in mediating viral replication and transcription.

3CLpro is a chymotrypsin-like cysteine ​​protease (~33 kDa) that is similarly termed the major protease due to its dominant role in processing the replicase polyprotein and genes.

Although 3CLpro is a dimer that provides a substrate-binding cleft, the protease exerts its proteolytic activity through nucleophilic attack directed by the cysteine ​​thiols in its Cys-His dimer.

There is a lack of evidence of 3CL pro's activity during the viral life cycle, so Pfizer researchers only consider 3CL pro as a potential primary drug target to bind and inhibit viral replication activity.

They need Simmons to judge from his perspective.

The 3CL pro structure is highly similar to the 3CL pro of different viral mutants, and inhibitors previously designed for MERS or SARS can also be effective against the current prevalent viruses.

At the same time, an inhibitor called N2, which was previously designed to inhibit 3CLSARS-CoV and MERS-CoV, was also tested against the virus this time.

Some researchers have proposed targeting 3CL pro, among which the efficacy of available drugs such as ritonavir, emedine, hesperidin, lopinavir, and indinavir have shown the potential to inhibit 3CL pro.

Green tea phytochemicals have also been evaluated intensively to identify those with the potential to inhibit 3CL pro.

In addition, phytochemicals from Neem were also evaluated against the target proteins of SARS-CoV-2, namely, membrane protein (M) and envelope protein (E), among which Nimocin, 24-Methylenecycloartan-3-one, phytosterols, Beta-Amyrin and Nimbolin A were injected into mice by Pfizer researchers.

Simmons touched each of the mice one by one. It was not known whether it was a psychological effect or a real reason, but he thought that he could make a better judgment by touching them with his own hands.

"I think this is just the best guinea pig here."

The researcher beside him quickly disinfected him, and another researcher asked: "This mouse was injected with a protease inhibitor."

"We developed protease inhibitors based on five prevalent M pro variants in the new coronavirus lineage, including G15S, T21I, L89F, K90R and L205V."

"I think we need more observation. We have just completed the initial injection. It is still unknown whether the mice injected with protease inhibitors can still maintain the best condition."

Wish you all a happy new year and all the best(*^_^*)
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(End of this chapter)